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Macaques are the most widely distributed nonhuman primates and used as animal models in biomedical research. The availability of full-genome sequences from them would be essential to both biomedical and primate evolutionary studies. Previous studies have reported whole-genome sequences from rhesus macaque Macaca mulatta and cynomolgus macaque M. Here, we present a fold coverage genome sequence of the Tibetan macaque M. TM is an endemic species to China belonging to the sinica group.

On the basis of mapping to the rhesus macaque genome, we identified approximately The heterozygosity rates of two CRs 0. Polymerase chain reaction resequencing of 18 TM individuals showed that 29 TSHNVs exhibited high allele frequencies, thus confirming their low heterozygosity. We further detected unusual low divergence regions between TM and CR. Given that TM and CR have overlapping geographical distributions, had the same refuge during the Middle Pleistocene, and show similar mating behaviors, it is highly likely that there was an ancient introgression event between them.

Moreover, demographic inferences revealed that TM exhibited a similar demographic history as other macaques until 0. Our study has provided new insight into the macaque evolutionary history, confirming hybridization events between macaque species groups based on genome-wide data. The Old World monkey genus Macaca is the most widespread nonhuman primates, having diversified 5—5. Macaques have long been used as important animal models in biomedical research because they are closely related to humans, sharing a last common ancestor about 25 Ma Kumar and Hedges

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